Figure 7.

In intracellular tachyzoites, Toxofilin distributes uniformly in the apical cytoplasm. Tachyzoites were incubated for 10 min (37°C, 5% CO2) with HFF cells plated on glass coverslips. The nonbound parasites were removed by changing the medium, and the infected cells were further incubated for 20 h as described in MATERIALS AND METHODS. At the end of the incubation period the cells were fixed, and extracellular tachyzoites were stained with the anti-P30 antibody followed by the anti-mouse antibody conjugated to Alexa-488, seen in red. The cells were subsequently permeabilized and stained for Toxofilin followed by an anti-rabbit conjugated to Alexa-568. Nuclei were stained by DAPI, shown here in green (artificial color). The actin cytoskeleton of the host cell was stained with rhodamine-phalloidin, shown in blue (artificial color). The indicated area was enlarged 2× and is presented as an inset.