Table 1.

F-actin quantitation

No. of cells analyzed	Expression of GFP-toxofilin	Expression of GFP	Mean fluorescence intensity (pixels)	% Control fluorescence intensity
29	+	−	1180 ± 250	55.8
42	−	−	2115 ± 582
33	−	+	1784 ± 311	107.0
39	−	−	1668 ± 416

The fluorescence associated with HeLa cells from several fields was analyzed using the NIH Image program. The area around each cell was delineated, and the mean fluorescence intensity was measured in pixels. The results are presented as the mean of the fluorescence intensity for each group of cells ± SD. Control, cells not expressing GFP or GFP-toxofilin.