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. 2000 Jan;11(1):369–391. doi: 10.1091/mbc.11.1.369

Table 4.

Mutations that rescue the cln1 cln2 cln3 strain

Mass at birth Mass at SBF 50% Mass at DNA repl. Mass at bud ini. Mass at division TG1 (min) Changed parameter Comments (Experimental results in boldface type)
1 wild type 0.71 1.07 1.15 1.15 1.64 84 CT 146 min
(daughter) (71′) (84′) (84′) (146′) (time of occurrence of event)
2 cln1 cln2 cln3 0.71 2.47 [6.20] [6.48] [8.11] Dn3 = 0 Richardson, 1989, inviable
ks,n2 = 0
3 cln1 cln2 cln3 0.65 0.65 0.75 0.95 1.50 25 Dn3 = 20 Schwob, 1993, Fig. 2, SBF activated soon
GAL-CLN3 ks,n2 = 0 after galactose induction
4 cln1 cln2 cln3 0.34 0.34 0.39 0.35 0.79 25 Dn3 = 0 Cross, 1991, Fig. 4, GAL-CLN1 induces cln2
GAL-CLN2 ks,n2 = 0.1
ks,n2 = 0
5 cln1 cln2 cln3 2.81 2.83 4.96 5.15 6.52 98 Dn3 = ks,n2 = 0 Epstein, 1992, viable
2X CLB5 ks,b5 = 0.012
ks,b5 = 0.04
6 cln1 cln2 cln3 1.23 2.07 2.05 2.14 2.86 88 Dn3 = ks,n2 = 0 Schwob, 1993, Fig. 6, viable
GAL-CLB5 ks,b5 = 0.1
ks,b5 = 0
7 cln1 cln2 cln3 0.71 2.57 [5.49] [6.64] [7.38] Dn3 = ks,n2 = 0 Amon, 1994, Fig. 8, G1 arrest
GAL-CLB2 ks,b2 = 0.1 We consider cells arrested in G1 if mass at
ks,b2 = 0  DNA replication exceeds 5
8 cln1 cln2 cln3 1.30 2.12 1.50 2.24 3.01 24 Dn3 = ks,n2 = 0 Tyers, 1996, Fig. 2, viable but large
 sic1 (85′) (24′) (94′) (146′) ks,c1 = 0
ks,c1 = 0
9 cln1 cln2 cln3 0.71 2.48 4.62 5.03 No mit Dn3 = ks,n2 = 0 Schwab, 1997, Fig. 3, M phase arrest
hct1 kd,b2 = 0.01
10 cln1 cln2 cln3 0.71 2.48 4.16 4.60 No mit Dn3 = ks,n2 = 0 Irniger, 1997, Fig. 2, M phase arrest
apc-ts kd,b2 = 0.01
kd,b2 = 0
kd,b5 = 0