Table 5.
Properties of bck2 mutants
| Genotype | Cell size relative to wild type
|
||||
|---|---|---|---|---|---|
| Observed
|
Simulation | ||||
| Epsteina | Di Comob | Dirickc | |||
| 1a | CLN1 CLN2 CLN3 BCK2 | 1 | 1 | 1 | 1 |
| b | bck2 | 1.3 | 1.5 | 1.8 | |
| c | + low-copy BCK2 | 0.8 | 0.7 with 4 copies | ||
| 2a | cln1 cln2 CLN3 BCK2 | 1.5 | 3.2 | 2.1 | |
| b | bck2 | 1.7 | 2.6 | ||
| 3a | CLN1 CLN2 cln3 BCK2 | 1.7 | 1.5 | 1.7 | 1.7 |
| b | bck2 | Inviable | Inviable | Inviable | |
| 4a | CLN1 CLN2 cln3 bck2 | ||||
| + GAL-CLN2 | Rescued | 0.5 | |||
| b | + GAL-CLB5 | 1.9 | |||
| c | + sic1 | 2.8 | |||
| d | + low-copy CLB5 | 3.7 with 10 copies | |||
| 5a | cln1 cln2 cln3 BCK2 | Inviable | Inviable | Inviable | |
| b | + low-copy BCK2 | Rescued | 3.9 with 4 copies | ||