Figure 2.

ATPase assays with Ssa1p. (A) Steady-state ATPase assay with Ssa1p, Sse1p, Fes1p, Ydj1p and combinations thereof. The ATPase rates are given relative to the basal activity of Ssa1p (set to one). (B) Single turnover analysis of ATP hydrolysis. [α-³²P]ATP–Ssa1p complexes were incubated with buffer or Sse1p (2 μM) or Fes1p (2 μM). The nucleotide status at different time intervals was analyzed by thin-layer chromatography. Shown is a plot of the ATP/(ATP+ADP) values from single turnover assays with Ssa1p alone, or in the presence of Sse1p or Fes1p over time.