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. 2006 May 11;25(11):2510–2518. doi: 10.1038/sj.emboj.7601139

Figure 5.

Figure 5

In vivo functions of Sse are essential. (A) Three tetrads generated by mating sse1Δ∷KAN and sse2Δ∷HIS strains were dissected, and viable spores assessed for markers on selective media. (B) Five-fold dilutions of sse1,2Δ cells expressing plasmid-borne SSE1, sse1-K69M or FES1 alleles were spotted onto selective or 5-FOA media (C) Western analysis of Sse1p and Ssa1p levels in W303, sse1Δ and sse1,2Δ strains expressing SSE1, sse1-K69M or FES1 alleles. sse1Δ samples have a small amount of crossreactive Sse2p. Ssa1p levels remain unchanged in all strains. Antibody recognizing glucose 6-phosphate dehydrogenase (Sigma) was a loading control. (D) sse1,2Δ strains expressing plasmid-borne SSE1, sse1-K69M or FES1 alleles were spotted in five-fold dilutions onto YPD media at permissive conditions (30°C), high temperature (37°C) or in the presence of hygromycin (10 μg/ml).