Figure 6.

Recovery of thermally denatured firefly luciferase (A, B) and bacterial luciferase (C, D). Cells were grown to mid-logarithmic phase and heat shocked as described in Materials and methods. All strains were assayed for a given substrate in the same experiment, however, are presented separately for clarity: (A, C) wild type, sse1Δ and fes1Δ, (B, D) sse1,2Δ strains expressing SSE1, sse1-K69M or FES1 alleles. Recovery of activity is expressed as a percentage of activity before heat treatments, and is the average of three independent samples. Error bars indicate the standard deviation.