Table 1.
PtdIns(3,4,5)P3 levels
| (A) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ssiPTEN | siPTEN | siPTEN+LY | LY | PTEN | PTEN C124S | PTEN C124S+LY | PTEN G129E | PTEN G129E+LY | |
| 1.00±0.01 | 2.84±0.14a | 1.00±0.01 | 0.63±0.02a | 0.35±0.01a | 3.33±0.21a | 1.02±0.02 | 3.30±0.20a | 1.01±0.02 | |
| (B) | |||||||||
| Insulin |
Insulin±LY |
Leptin |
Leptin±LY |
D3PTEN |
D3PTEN±LY |
A3PTEN |
A3G129E |
D3G129E |
D3G129E±LY |
| 3.50±0.10a | 1.02±0.02 | 3.24±0.10a | 0.98±0.01 | 3.02±0.09a | 1.02±0.02 | 0.42±0.02a | 2.64±0.11 | 3.12±0.13a | 1.02±0.02 |
| Levels of PtdIns(3,4,5)P3 in N29/4 cells following treatment with PTEN constructs. Data are shown as a ratio of control (untreated) cells. Cells treated with 10 μM LY294002 for 20 min before fixing. | |||||||||
| aP<0.05 compared with control. | |||||||||