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. 2006 May 11;25(11):2551–2563. doi: 10.1038/sj.emboj.7601136

Figure 4.

Figure 4

APCCdh1-mediated degradation of microtubule-associated proteins prevents spindle assembly. (A) (Left panel) cdc28Y19E cdh1Δ cells or cdc28Y19E cdc20-1 were arrested with α-factor and released at 37°C. (Right panel) cdc28-as1 cells with similar mutations were released in the presence of 1NM-PP1. Cells are shown 240 min after release. (B) cdc28-as1 and cdc28-as1 cdh1Δ cells carrying endogenously tagged Cin8-HA3, Kip1-HA3 or Ase1-HA3 were arrested in telophase at 37°C by a cdc15-2 mutation, and released in the presence of 1NM-PP1 at 24°C. (C) G1-synchronised WT cells tagged endogenously with Cin8-HA3 with or without GAL-Cdh1-cmyc3 on a CEN plasmid were released from YEP+Raff into YEP+Raff+Gal medium. (D) cdc15-2 and cdc15-2 cdh1Δ cells expressing Spc29-CFP were synchronized in telophase at 37°C, released into fresh medium at 24°C and were monitored for kinetics of spindle assembly. ‘*'Indicate the time at which a significant difference in the percentage of cells-with-spindles was observed between the two strains.