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. 2006 May 11;25(11):2347–2357. doi: 10.1038/sj.emboj.7601137

Figure 2.

Figure 2

EPS-8 expression is upregulated in the 1° vulval cells. Expression of the eps-8p::gfp transcriptional reporter in the VPCs or their descendants of (A) a wild-type larva at the Pn.p cell stage (early L3), (B) at the Pn.px, (C) at the Pn.pxx stage (both mid L3) and (D) at the L4 stage. (E) Whole-mounts of wild-type larvae at the Pn.p cell stage and (F) at the L4 stage stained with polyclonal EPS-8 antibodies (in green). Adherens junctions are stained with MH27 in red. (G) eps-8p::gfp expression in a let-60(n1046gf) and (H) lin-7(e1413) mutant at the Pn.p stage (early L3). (I) eps-8p::gfp expression in a gonad-ablated L3 larva lacking the AC at the Pn.p stage (early L3). For a quantification of the expression patterns, see Supplementay Figure S1. Scale bar in (I) is 10 μm.