Table 1.
eps-8 positively regulates EGFR/RAS/MAPK signaling
| Row | Genotype | Induced VPCsa | % Vulb | % Muvc | n | P-valued |
|---|---|---|---|---|---|---|
| 1 | Wild-type | 3.0 | 0 | 0 | Many | — |
| 2 | eps-8(lf) | 3.0 | 0 | 0 | 35 | — |
| 3 | [lin-31p::eps-8a] | 3.1 | 0 | 16 | 131 | <0.0001 (1) |
| 4 | lin-3(rf) | 0.8 | 95 | 0 | 75 | — |
| 5 | lin-3(rf); gfp RNAi | 0.5 | 88 | 0 | 128 | — |
| 6 | lin-3(rf); eps-8 RNAi | 0.2 | 98 | 0 | 97 | <0.005 (5) |
| 7 | lin-3(rf); [lin-31p::eps-8a] | 2.1 | 35 | 0 | 71 | <0.005 (4) |
| 8 | let-23(rf) | 1.1 | 80 | 0 | 106 | — |
| 9 | let-23(rf); eps-8(lf) | 0.4 | 97 | 0 | 32 | <0.0001 (8) |
| 10 | let-23(rf); [lin-31p::eps-8a] | 3.0 | 0 | 0 | 38 | <0.0001 (8) |
| 11 | lin-7(lf) | 0.9 | 94 | 0 | 72 | — |
| 12 | lin-7(lf); eps-8(lf) | 0.3 | 94 | 0 | 33 | <0.001 (11) |
| 13 | lin-7(lf); gfp RNAi | 0.9 | 95 | 0 | 73 | — |
| 14 | lin-7(lf); eps-8 RNAi | 0.3 | 98 | 0 | 71 | <0.001 (13) |
| 15 | lin-7(lf); [lin-31p::eps-8a] | 3.0 | 0 | 0 | 35 | <0.0001 (11) |
| 16 | lin-2(lf) | 0.6 | 92 | 3 | 38 | — |
| 17 | lin-2(lf); eps-8(lf) | 1.0 | 77 | 0 | 26 | <0.25 (16) |
| 18 | lin-2(lf); [lin-31p::eps-8a] | 1.8 | 73 | 3 | 30 | <0.0001 (16) |
| 19 | sem-5(rf) | 1.1 | 75 | 0 | 64 | — |
| 20 | sem-5(rf); [lin-31p::eps-8a] | 1.1 | 74 | 0 | 27 | <0.95 (19) |
| 21 | let-60(gf) | 4.5 | 0 | 84 | 44 | — |
| 22 | let-60(gf); eps-8(lf) | 3.3 | 0 | 25 | 20 | <0.0001 (21) |
| 23 | let-60(gf); gfp RNAi | 4.9 | 0 | 90 | 101 | — |
| 24 | let-60(gf); eps-8 RNAi | 3.7 | 0 | 64 | 75 | <0.0001 (23) |
| 25 | [hs::mpk-1]e | 4.5 | 0 | 69 | 24 | |
| 26 | [hs::mpk-1]; eps-8(lf)e | 3.0 | 0 | 0 | 28 | <0.0001 (25) |
| 27 | [hs::mpk-1]; gfp RNAie | 4.3 | 0 | 70 | 40 | — |
| 28 | [hs::mpk-1]; eps-8 RNAie | 3.4 | 0 | 23 | 49 | <0.0001 (27) |
| 29 | lin-12 (n302) | 0.4 | 92 | 0 | 37 | |
| 30 | lin-12 (n302); eps-8(lf) | 0.7 | 96 | 0 | 27 | <0.2 (29) |
| 31 | lin-12 (n379) | 0.5 | 90 | 0 | 42 | |
| 32 | lin-12 (n379); eps-8(lf) | 0.3 | 100 | 0 | 45 | <0.2 (31) |
| Alleles used: zhIs11[lin-31::eps-8a], eps-8(by160); Ex[opt-2::eps-8A], lin-3(e1417), let-23(sy1), lin-7(e1413), lin-12(n302); lin-12(n379), lin-2(n397), sem-5(n2019), let-60(n1046gf), gaIs36[hs::mpk-1(+), D-mek-2(gf)]. | ||||||
| aThe induction index indicates the average number of VPCs per animal that adopted 1° or 2° vulval fates as described (Berset et al, 2001). | ||||||
| b%Vul indicates the fraction of animals with fewer than three induced VPCs. | ||||||
| c%Muv indicates the fraction of animals with more than three induced VPCs. | ||||||
| dStatistical significance was tested with two-tailed Student's t-test. Numbers in parentheses indicate the row to which a data set was compared. | ||||||
| eTo induce MPK-1 expression, L1 larvae were heat-shocked for 30 min at 33°C every 24 h until the L4 larval stage. | ||||||