Figure 2.

Quantification and visualization of surface dynamics for speckles in transcriptionally active cells. (A) A single frame from the video sequence (Video 1) shows highly variable morphology of nuclear speckles. (B) Quantification of surface velocities. For display reasons velocities for eight representative of a total of 45 speckles for this cell are shown. For each speckle surface velocities are calculated from the average velocity of corresponding boundary points in adjacent time sections. Note that b-spline interpolation, which does not tend to oscillations even for a large number of sample points, was chosen for time-space reconstruction of speckles. (C) Spatiotemporal reconstruction of speckles (Video 2). (D) Detail from C.