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. 2006 May 18;25(11):2564–2574. doi: 10.1038/sj.emboj.7601141

Figure 2.

Figure 2

Effect of sws1+ deletion on survival of srs2Δ and rqh1Δ mutants. (A) Spot assay of wt (PR109), sws1Δ (VM3723), srs2Δ (VM3724) and sws1Δ srs2Δ (VM3721) strains. Four-fold serial dilutions of each strain were plated on YES or YES supplemented with 6 μM camptothecin. Photographs were taken after 4 days at 32°C. (B) DNA damage sensitivity of wt (PR109), sws1Δ (VM3723), rqh1Δ (SC3250), and sws1Δ rqh1Δ (VM3722) cells. Four-fold serial dilutions were plated onto YES plates and treated with the indicated DNA damaging agents. Photographs were taken after 4 days at 32°C. (C) Percentages of dividing cells with ‘cut' phenotype observed in wt (PR109), sws1Δ (VM3723), srs2Δ (VM3724), rqh1Δ (SC3250), sws1Δ srs2Δ (VM3721) and sws1Δ rqh1Δ (VM3722) strains at the indicated time points after release from 5-h-incubation in YES supplemented with 12 mM HU.