Figure 4.

Spontaneous Rad22-YFP foci formation and recombination levels are reduced in sws1Δ background. (A) Cells containing a genomic copy of Rad22-YFP were grown in YES media at 32°C until mid-log phase and their Rad22-YFP foci were quantified. For each strain more than 300 cells were counted. The average percentage of nuclei containing at least one focus is shown. The strains used in this assay were wt (VM3725), sws1Δ (VM3729), rqh1Δ (VM3726), sws1Δrqh1Δ (VM3730), srs2Δ (VM3727), sws1Δsrs2Δ (VM3728). (B) Comparison of the spontaneous recombination frequencies of wt (PS3), sws1Δ (VM3731), rqh1Δ (VM3732) and sws1Δ rqh1Δ (VM3736). Strains containing an ura4⁺ marker flanked by two ade6⁻ heteroalleles were used in this analysis. Recombination frequencies are mean values from three independent assays.