Figure 10.

Microtubule gliding and endocytic vesicle translocation are oscillatory events. The instantaneous velocities from two representative microtubules and two vesicles are shown. Microtubule gliding was powered by motor protein isolated from rat liver, and endocytic translocation was powered by endosomes isolated from rat liver (without exogenous motor protein). The gliding of minus-end-directed microtubules (A) showed larger velocity amplitudes with shorter pauses compared with plus-end gliding (B). Translocating vesicles (C and D) showed smaller velocity amplitudes and shorter peak-to-peak intervals compared with gliding microtubules (A and B).