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Influence of microtubule attachment method on parameters of endocytic vesicle motility
| Microtubule attachment method | Motile vesicles (% of total) | Minus-end-directed movement (% of total movement) | Velocity (μm/s) |
|---|---|---|---|
| DEAE-dextran | 33 (174) | 49 (39) | 0.71 ± 0.44 (10) |
| DEAE-dextran plus vesicle preincubation with exogenous motor proteins | 39 (122) | 45 (31) | 0.71 ± 0.36 (10) |
| Motor proteins | 27 (199) | 47 (43) | 0.55 ± 0.23 (21) |
The number of vesicles counted is in parentheses.
