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. 2000 Feb;11(2):435–452. doi: 10.1091/mbc.11.2.435

Figure 3.

Figure 3

Multicopy suppressors of chs5 spa2 lethality at 37°C. chs5 spa2 strain (Y1941) transformed with vector (YEp24) or plasmids containing CHS5, SPA2, or the suppressor genes CSR1, CSR2, CSR3, or CSR4 were grown overnight at 30°C in SC-Ura plus 1 M sorbitol. Ten-fold serial dilutions starting with 10% of the saturated culture were spotted in 5-μl drops onto SC-Ura plates (top panel) or SC-Ura plus sorbitol (bottom panel) and were incubated at 37°C. The suppressor genes rescue the growth defect of chs5 spa2 cells to different degrees.