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sbe2 sbe22 mutants are sensitive to calcofluor and SDS. Wild-type (Y270), sbe2 (Y1942), sbe22 (Y1943), and sbe2 sbe22 (Y1944) homozygous diploid strains were grown overnight at 30°C in YPDAS. (A) Five-fold serial dilutions starting with 20% of the saturated culture were spotted in 5-μl drops onto SC and SC containing 50 μg/ml calcofluor. (B) Ten-fold dilutions starting with the saturated culture were spotted onto YPDA and YPDA containing 0.0025% SDS. All plates were incubated at 30°C.
