Figure 9.

sbe2 sbe22 diploid cells display a random budding pattern. Wild-type (Y270), sbe2 (Y1942), sbe22 (Y1943), and sbe2 sbe22 (Y1944) diploid cells were stained with calcofluor to visualize bud scars. (A) In wild-type diploid cells, bud scars accumulate at both ends of the cell. In contrast, sbe2 sbe22 diploids exhibit a random budding pattern. (B) Cells with two or more bud scars were scored and classified as described in MATERIALS AND METHODS. Percentages of cells in each category are shown. n, number of cells scored.