Figure 7.

Immunogold labeling of cells expressing the EGFP-Rab7 wt. The panels show examples of large vesicular structures forming tightly packed aggregates. These structures appear as multivesicular bodies with numerous small, internal vesicles, or they have a more typical lysosome appearance with a dense content of membranous material. Note that all of these aggregated, late endosome/lysosome–like structures (Ly) are distinctly labeled for EGFP (10-nm gold; arrowheads) on the cytoplasmic surface of their outer membranes, as well as for Lamp-1 internally (15-nm gold, small arrows). Also note that very little cytosolic labeling for EGFP is seen. Bar, 250 nm.