Figure 3.

Relative distribution of endogenous dynamin between the plasma membrane and the intracellular compartments. Ultracryosections from three different HeLa dynK44A cultures (at least two passages apart) grown in the presence of tetracycline were labeled with the hudy-1 antibody. Gold particles (5 nm) associated with or <100 nm away from the plasma membrane, including clathrin-coated pits (PM), Golgi stacks/TGN (Golgi), vacuolar and tubulo-vesicular endosomes (Endos.), and the cytosol, including unidentified cytosolic objects (Cytosol), were counted on randomly photographed EM pictures (magnification, ×84,000). A total of 8018 gold particles were scored. Values are means ± SE (n = 3).