Figure 8.

Expression of mutant dynamin causes a redistribution of the CI-MPR to an endocytic compartment strongly positive for Lamp-1 but distinct from classic lysosomes. The frequency of types 1–6 endocytic structures in control cells and cells expressing mutant dynamin was compared based on immunogold double-labeling experiments and the extent of CI-MPR/Lamp-1 colocalization, as described in Figure 7 (bars, mean ± SE, n = 3). Black dots represent the distribution of CI-MPR among the compartments (mean ± SE, n = 3). The material analyzed includes 800 endocytic organelle profiles pooled from three independent experiments with control cells and 614 endocytic organelle profiles from three independent experiments with cells expressing mutant dynamin.