Figure 5.

Western blot analysis of yl mutants. Western analysis of ovary proteins using anti-Yl antibodies shows that the 210-kDa Yl protein is absent in yl alleles with a strong phenotype (yl¹¹,yl¹⁴, yl²⁸), but it is present in other alleles with strong reductions in yolk uptake (yl¹⁵,yl²⁰, yl²¹) as well as in alleles with weak phenotypes (yl¹⁸). Two alleles, yl¹⁶ and yl²⁹, expressed truncated proteins of 130 kDa (*) and 175 kDa, respectively. The low-molecular-weight band is a cross-reacting protein. It is not detected using affinity-purified anti-Yl antibodies; however, it serves as a useful loading control.