Figure 3.

Sequence alignment of the microtubule contact site for several cytoplasmic dyneins and MAP1B. The comparison begins at the conserved proline (aa 3366 in Dd) that is thought to mark the end of the first helical region and ends at proline 3491 that begins the second helical region. The Dictyostelium sequence is shown on top; the characters above mark those residues changed to alanine. +, enhanced binding; −, no binding; w, weak binding; o, no effect; P, poor extraction with ATP. Dd, Dictyostelium discoideum; Rn, Rattus norvegicus; Dm, Drosophila melanogaster; Ce, Caenorhabditis elegans; Nh, Nectria hematococca; Nc, Neurospora crassa; An, Aspergillus nidulans; Sc, Saccharomyces cerevisiae; Pt, Paramecium tetraurelia; Sp, Schizosaccharomyces pombe.