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. 2006 Jun 16;2(6):e94. doi: 10.1371/journal.pgen.0020094

Figure 6. Overexpression of Xist cDNA Transgene and Impact of Interference on ncRNAs and Imprinted RNAs.

Figure 6

(A–B) Quantification of Xist and Tsix RNAs using Xist exon1–exon3 and Tsix using exon2–exon3 primers in the parental female ES cell line (LF2) and knocked down clones for Eif1 (E1), Rent1 (R1), and Exosc10 (Ex1), and in the same cells 48 h after transfection with Xist cDNA transgene. Quantification of His1 (C), Bc1 (D), Ube3A (E), Peg3 (F), and H19 (G) RNAs using His1Up/Lo, Bc11Up/Lo, Ube3AUp/Lo, Peg3Up/Lo, and H19Up/Lo primers respectively, at d 0 and d 4 of retinoic acid–induced ES cell differentiation.

Columns and bars are constructed as in Figure 1.