. 2006 Apr 7;91(1):244–254. doi: 10.1529/biophysj.106.082495

TABLE 1.

Composition of solutions

Extracellular solutions (mM)	NaCl	NaMs	KCl	KMs	NMDG-Cl	NMDG-Ms	MgCl₂	MgMs₂	K₂EGTA	Na₂EGTA	NaH₂PO₄	HEPES
Ca-free Tyrode's	135	0	5.4	0	0	0	1.0	0	0.1	0	0.33	10
Mg-loading	0	0	5.4	0	101	0	17.9	6.0	0.1	0	0.33	10
75 K-70 Na	3.5	66.2	5.4	65.3	0	0	1.0	0	0.1	0	0.33	10
0 K-70 Na	69.5	0	0	0	70.3	0	1.0	0	0	0.1	0.33	10
0 K-70 Na-0 Cl	0	69.5	0	0	0	70.3	0	1.0	0	0.1	0.33	10
0 K-0.5 Na	0	0	0	0	135	0	1.0	0	0	0.1	0.33	10
0 K-0.5 Na-0 Cl	0	0	0	0	0	135	0	1.0	0	0.1	0.33	10
Pipette solutions (mM)	KMs	CsMs	NaCl	CsCl	CaCl₂	CaMs₂	MgCl₂	MgMs₂	MOPS	[K⁺]	[Na⁺]	[Cl⁻]
135 K-10 Na	130	0	10	0	1.0	0	1.0	0	10	135	10	14
145 Cs	0	130	0	10	1.0	0	1.0	0	10	0	0	14
145 Cs-0 Cl	0	140	0	0	0	1.0	0	1.0	10	0	0	0

Ms, methanesulfonate; NMDG-Cl, N-methyl-D-glucamine titrated by HCl; NMDG-Ms, N-methyl-D-glucamine titrated by methanesulfonic acid. Extracellular solutions also contained 5 mM glucose in common. The pH of the extracellular solutions was adjusted to 7.40 by adding NaOH (Ca-free Tyrode's), NaOH plus HCl (Mg-loading), KOH (75 K-70 Na), HCl (0 K-70 Na and 0 K-0.5 Na) or HMs (0 K-70 Na-0 Cl and 0 K-0.5 Na-0 Cl). Final Na⁺ concentrations were 140 mM (Ca-free Tyrode's), 1.6 mM (Mg-loading), 70 mM (75 K-70 Na, 0 K-70 Na, and 0 K-70 Na-0 Cl) and 0.5 mM (0 K-0.5 Na and 0 K-0.5 Na-0 Cl). The pH of the pipette solutions was adjusted to 7.15 with either 5 mM KOH (135 K-10 Na) or 5 mM CsOH (145 Cs and 145 Cs-0 Cl). The three rightmost columns under Pipette solutions give the final concentrations of K⁺, Na⁺, and Cl⁻.