FIGURE 5.

(A) Contributions to ΔΔG^‡ from (green line), (red line), and (blue line; dashed line, Ac_n, and dotted line, Hex_n). The sum of the electrostatic contributions is marked as the red-green dashed line. The data for ΔΔG^‡—the sum of the four components—are shown for BCA-Ac_n (▪) and BCA-Hex_n (○). The fits to the data (dashed line, BCA-Ac_n; dotted line, BCA-Hex_n) are those given by Eq. 9. See text for details of the model. (B) An example of how the energy of the transition state changes relative to that of the ground state with 10 modifications. The folded states of BCA, BCA-Ac₁₀, and BCA-Hex₁₀ are scaled to the same energy. The arrows (in the same colors as A) indicate how each of the factors changes the relative position of the transition state. The red/green dashed line shows the effects of just the electrostatic terms on the energy of the transition state (i.e., if 10 lysine groups were neutralized with no corresponding change in hydrophobicity).