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(A) ECFP-Cdc20 expressing PTK2 cell in early G1 phase. (B) ECFP-Cdc20 expressing PTK2 cell in interphase. (C) The autocorrelation functions from the FCS measurements on interphase cells (G2 and early G1 phase) are fitted with a single species model (bold solid lines), which reveals that Cdc20 is a stable component of a large complex in interphase cells with a diffusion constant of D = 1.8 ± 0.1 μm2/s.
