Figure 8.

TX-100 insolubility of PLAP and NTR-PLAP in FB1- or mevinolin (Mev)- and M-β-CD–treated cells. FRT cells expressing PLAP and NTR-PLAP were grown on dishes in the absence or presence of FB1 or mevinolin and M-β-CD, as described in MATERIALS AND METHODS. Cells were then labeled overnight, lysed in buffer containing 1% TX-100, and soluble and insoluble fractions were separated by centrifugation and immunoprecipitated with antibodies against PLAP and NTR-PLAP. Bars represent TX-100–soluble fractions as percentage of total protein. Although FB1 does not alter TX-100 behavior of PLAP and NTR-PLAP, the continued treatment with mevinolin and M-β-CD increases the TX-100 solubility of both proteins.