FIG. 1.

Confocal microscopic image of a mature C. neoformans biofilm treated with capsular binding MAb 18B7. Orthogonal images of a mature C. neoformans biofilm show capsular binding MAb 18B7 (blue; GAM-γ1-AF) penetration within internal regions of a biofilm (A); metabolically active (red; FUN-1-stained) C. neoformans cells (B); extracellular polysaccharide material (green; ConA stained) (C); and a superimposition of panels A, B, and C (D). The thickness of mature biofilms is approximately 97 μm. Arrows denote the locations of MAb 18B7 in a mature cryptococcal biofilm. Pictures were taken using a 40× power field. Scale bars, 50 μm.