FIG. 3.

Inhibition of HCoV-NL63 by the HR2 peptide. (A) SDS-polyacrylamide gel electrophoresis analysis of HR peptides separately and the HR1/HR2 complex formation. The molecular mass of the complex corresponds to the predicted heterohexamer. (B) CPE reduction mediated by HR2 peptide. Filled circles indicate CPE development, empty circles indicate the absence of CPE, and the half-filled circle represents the development of CPE in 50% of wells. (C) Decrease in HCoV-NL63 virus yield after HR2 treatment and cell viability assay. Numbers on the y axis represent the percentages of produced virus and percentages of viable cells. (D) Immunostaining-based HCoV-NL63 infection inhibition assay. Values on the y axis represent the percentages of infected cells.