TABLE 1.
Test compound | Mol wt | Highest nontoxic dosea (μM) | % Inhibition (mean ± SD)b
|
Approximate IC50c (μM)
|
||
---|---|---|---|---|---|---|
E. intestinalis | V. corneae | E. intestinalis | V. corneae | |||
Fumagillin | 458.60 | 4 | 72.1 ± 2.8 | 72.8 ± 3.1 | 0.0025 | 0.002 |
TNP-470 | 401.88 | 50 | 92.1 ± 1.6 | 90.4 ± 4.3 | 0.0035 | 0.002 |
Ovalicin | 296.36 | 10 | 73.0 ± 14.9 | 78.9 ± 8.5 | 0.004 | 0.002 |
9168d | 458.54 | >100e | 71.7 ± 13.0 | 78.2 ± 19.1 | 0.02 | 0.42 |
9665d | 282.38 | >100e | 74.3 ± 30.1 | 93.4 ± 28.6 | 0.001 | 0.066 |
56407d | 192.17 | >100e | 70.6 ± 12.3 | 54.9 ± 5.0 | 8.90 | 57.00 |
58368d | 639.86 | 10 | 58.7 ± 10.5 | 75.5 ± 10.7 | 6.25 | 0.20 |
141538d | 296.36 | 10 | 58.0 ± 9.9 | 54.1 ± 8.9 | 0.75 | 8.20 |
141539d | 298.38 | 10 | 83.3 ± 8.9 | 66.8 ± 17.7 | 0.07 | 0.08 |
676019d | 431.00 | 10 | 29.5 ± 6.1 | 28.0 ± 14.0 | 50.4 | 97.0 |
174554d | 241.28 | >100e | 80.8 ± 11.6 | 91.2 ± 3.3 | 47.1 | 52.9 |
Concentrations of compounds that caused the viability of host cells (RK-13 cells) to fall below 85% of medium-treated host cells were considered to be toxic.
Inhibition values were presented for the highest nontoxic dose of compound.
IC50 values were estimated by interpolation using Graphpad Prism software.
The numbers for these compounds refer to National Cancer Institute database NSC entry numbers, and additional information can be accessed at the website http://dtp.nci.nih.gov/dtpstandard/ChemData/index.jsp.
The highest concentration tested for each compound was 100 μM, and these compounds were not toxic at this dose.