TABLE 2.
Microsporidium-associated lesions in treated V. corneae-infected athymic mice
| Treatment | Mean no. of lesions (± SD)a
|
||
|---|---|---|---|
| Small intestine | Liver | Pancreas | |
| V. corneae only | 74.0 ± 56.7 | 39.9 ± 20.3 | 18.1 ± 19.8 |
| V. corneae + fumagillin (5 mg/kg s.c.) | 18.3 ± 15.6 (P < 0.01)b | 21.8 ± 30.0 | 4.6 ± 6.3 (P < 0.05) |
| V. corneae + fumagillin (10 mg/kg s.c.) | 8.6 ± 10.7 (P < 0.01) | 13.5 ± 18.9 | 1.6 ± 2.0 (P < 0.05) |
| V. corneae + fumagillin (20 mg/kg s.c.) | 9.5 ± 13.2 (P < 0.01) | 27.3 ± 22.0 | 1.4 ± 2.8 (P < 0.05) |
| V. corneae only | 53.5 ± 49.1 | 25.0 ± 21.2 | 12.4 ± 15.2 |
| V. corneae + TNP-470 (10 mg/kg i.p.) | 19.1 ± 11.9 | 29.9 ± 36.7 | 13.3 ± 13.4 |
| V. cornae + TNP-470 (20 mg/kg i.p.) | 6.3 ± 8.5 (P < 0.05) | 17.9 ± 18.8 | 1.6 ± 3.3 |
| V. corneae only | 53.5 ± 49.1 | 25.0 ± 21.2 | 12.4 ± 15.2 |
| V. corneae + ovalicin (5 mg/kg s.c.) | 11.6 ± 9.5 (P < 0.05) | 17.9 ± 16.7 | 6.8 ± 6.3 |
| V. corneae + ovalicin (10 mg/kg s.c.) | 17.3 ± 14.2 | 12.8 ± 7.3 | 10.0 ± 7.9 |
| V. corneae + ovalicin (20 mg/kg s.c.) | 24.9 ± 25.1 | 22.3 ± 15.7 | 15.9 ± 12.5 |
| V. corneae only | 30.4 ± 16.9 | 14.6 ± 14.8 | 31.0 ± 30.3 |
| V. corneae + NSC 9665 (5 mg/kg i.p.) | 15.3 ± 13.3 | 9.6 ± 4.9 | 43.0 ± 18.4 |
| V. corneae + NSC 9665 (10 mg/kg i.p.) | 8.3 ± 7.2 (P < 0.01) | 15.7 ± 17.8 | 18.9 ± 25.2 |
| V. corneae + NSC 9665 (20 mg/kg i.p.) | 3.5 ± 5.0 (P < 0.001) | 20.8 ± 12.7 | 19.5 ± 15.0 |
| V. corneae only | 25.3 ± 12.8 | 25.0 ± 14.0 | 13.0 ± 7.4 |
| V. corneae + NSC 141539 (5 mg/kg i.p.) | 5.5 ± 5.7 (P < 0.05) | 36.7 ± 29.6 | 5.3 ± 5.6 |
| V. corneae + NSC 141539 (10 mg/kg i.p.) | 22.0 ± 12.5 | 18.9 ± 12.8 | 46.9 ± 22.6 |
| V. corneae + NSC 141539 (20 mg/kg i.p.) | 14.8 ± 9.2 | 25.3 ± 14.8 | 32.4 ± 23.8 |
a
Groups of eight mice each were inoculated i.p. with 1 × 107 V. corneae microsporidia, and drug treatments were administered daily beginning 24 h later. Microsporidium-associated lesions were counted in one cross-section of each tissue per mouse as described in Materials and Methods.
b
Values of each drug treatment group and the untreated group of that experiment were compared. Statistically significant differences were determined by Student's t test.