TABLE 5.
Summary of the typical procedures of purification of BJP-1 metallo-β-lactamase produced in E. coli BL21(DE3)[pET-BJP]
| Product of purification step | Vol (ml) | Total amt of protein (mg) | Total activity (U)a | Sp act (U/mg protein) | Recovery (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| Cell extract | 90 | 2,000 | 40,860 | 21 | 100 | 1 |
| DEAE Sepharose FTb | 160 | 109 | 27,500 | 252 | 67 | 12 |
| Source Q FT | 55 | 50 | 25,355 | 506 | 61 | 24 |
a
One unit of activity is defined as the amount of enzyme hydrolyzing 1 μmol of cephalothin per min under the conditions described in Materials and Methods.
b
FT, flowthrough.