TABLE 1.

Primers used for mapping HPV-16 E1 and E2 genes and detection of beta-globin target regions

Primer	5′ to 3′ sequence	Locationa	Size (bp)
E1F1	CAGGTACCAATGGGGAAGA	878-896
E1R1	ACCTGTACTGCATCTCTATGTTGT	1095-1118	241
E1F2	GACAGCACATGCGTTGTTTAC	1059-1079
E1R2	AGTCTCATGGCGCCCTTC	1306-1323	265
E1F3	CGCCATGAGACTGAAACAC	1312-1330
E1R3	GAAAAACTCACCCCGTATAAC	1503-1523	212
E1F4	GTTATACGGGGTGAGTTTTTC	1503-1523
E1R4	ATCATCATACACATTGGAGACA	1763-1784	282
E1F5	GTGTGTCTCCAATGTGTATGATG	1760-1782
E1R5	GCAATTTCACTATCGTCTACTATG	1968-1991	232
E1F6	GGGCCTACGATAATGACATAGTA	1952-1974
E1R6	TGCTTCCAATCACCTCCAT	2168-2186	235
E1F7	GGAGGTGATTGGAAGCAA	2170-2187
E1R7	CCAATGCATTTCTTAAATTGTC	2464-2485	316
E1F8	CCCTGTTGGAACTACATAGATGAC	2443-2466
E1R8	CCTCGTCCTCGTGCAAACT	2725-2743	301
E1F9	CCAGTGTATGAGCTTAATGATAAGA	2662-2686
E2R9	GTCTATATGGTCACGTAGGTCTGTA	2824-2848	187
E2F10	GTGTGTCAGGACAAAATACTAACA	2783-2806
E2R10	AAATACACTTCAAGGCTAACGTC	3041-3063	281
E2F11	GAAAAGTGGACATTACAAGACGTTA	3023-3047
E2R11	CTGCACAAAATATGTTCGTATTC	3243-3265	243
E2F12	GAATACGAACATATTTTGTGCAG	3243-3265
E2R12	CTGAGTCTCTGTGCAACAACTTA	3517-3539	297
E2F13	CTAAGTTGTTGCACAGAGACTCA	3516-3538
E2R13	GACGACACTGCAGTATACAATGTAC	3681-3705	190
E2F14	CATGGCATTGGACAGGACATA	3708-3728
E5R14	ACGCCAGTAATGTTGTGGAT	3870-3889	182
PCO3	ACACAACTGTGTTCACTAGC	62150-62169
PCO4	CAACTTCATCCACGTTCACC	62240-62259	110
Probe	TAACGGCAGACTTCTCCTCAGGAGTCAGG	62195-62223
GlobF	GATGCCCTCTCTCACCACTC	71147-71166
GlobR	AATGGTCATACTGCCCAAGG	71609-71619	473
E6FqPCR	GAGAACTGCAATGTTTCAGGACC	94-115
E6RqPCR	TGTATAGTTGTTTGCAGCTCTGTGC	150-174	81
E6 Probe	CAGGAGCGACCCAGAAAGTTACCACAGTT	119-147
E2FqPCR	AACGAAGTATCCTCTCCTGAAATTATTAG	3362-3390
E2RqPCR	CCAAGGCGACGGCTTTG	3427-3443	82
E2 Probe	CACCCCGCCGCGACCCATA	3407-3425

^aNucleotide positions based on the revised, or HPV-16R, sequence (20, 22) and GenBank accession no. U01317 for beta-globin. Primers used to detect HPV-16 E6 and E2 by qPCR were described elsewhere (24).