Figure 1.

Deletion of cig1⁺, cig2⁺, and puc1⁺ causes a cell cycle delay in G1. (A) Flow cytometry analysis of the wild type and cig1, cig2, and puc1 single, double, and triple mutants in wild-type and wee1-50 backgrounds at 25°C. (B) Flow cytometry analysis of wild type, cig1Δ cig2Δ puc1Δ, wee1-50, and wee1-50 cig1Δ cig2Δ puc1Δ at 35°C. For each strain, there is a histogram (left) and a dot-plot (right) representation of the data. The vertical lines in the dot plots correspond to the biggest cells in the wild-type culture. (C) Quantification of the data shown in A and B to indicate the percentage of cells in G1 in the different cyclin mutants. (D) Cell size of the different cyclin mutants in arbitrary units considering 100 as the size of the wild type, wee1-50 at 25°C, or wee1-50 at 36°C. Cells were grown in minimal medium to midexponential phase.