Figure 3.

Rum1 protein persists for longer in the cig1Δ cig2Δ puc1Δ mutant than in the wild type. Wild-type and cig1Δ cig2Δ puc1Δ mutant cells were nitrogen starved for 8 h, and then nitrogen was added back to the culture. Samples were taken for protein extracts and flow cytometry at the indicated times. (A) Cdc13, rum1, and cdc2 protein levels in wild-type and cig1Δ cig2Δ puc1Δ cells. +N corresponds to cells growing in minimal medium. (B) Cdc2/cdc13 kinase assays. Cdc2/cdc13 complexes were immunoprecipitated with anti-cdc13 antibodies and assayed with the use of histone H1 as substrate. (C) Flow cytometry analysis of wild-type and cig1Δ cig2Δ puc1Δ mutant cells during the release from nitrogen starvation.