TABLE 2.

Probes used in this study

Drug	Target gene	Probe namea	Sequenceb	Temp (°C)	% GC
Rifampin	rpoB	RMP-W511L	5′-T15-CAGCCAGCTGAGCCAATTCA-3′	54	67
		RMP-M511P	5′-T15-AGCCAGCCGAGCCAA-3′	47	55
		RMP-W513Q	5′-T15-CTGAGCCAATTCATG-3′	39	47
		RMP-M513L	5′T15-CTGAGCCTATTCATGG-3′	43	50
		RMP-W516D	5′-T15-TTCATGGACCAGAACA-3′	41	44
		RMP-M516V	5′-T15-TTCATGGTCCAGAACA-3′	41	44
		RMP-M516Y	5′-T15-ATTCATGTACCAGAACA-3′	40	35
		RMP-W522S	5′-T15-CTGTCGGGGTTGAC-3′	43	64
		RMP-M522L	5′-T15-CTGTTGGGGTTGAC-3′	40	57
		RMP-W526H	5′-T15-GTTGACCCACAAGCGCCGA-3′	55	63
		RMP-M526Y	5′-T15-GGTTGACCTACAAGCGC-3′	49	59
		RMP-M526D	5′-T15-GGTTGACCGACAAGCGC-3′	52	65
		RMP-M526R	5′-T15-TTGACCCGCAAGCGC-3′	47	67
		RMP-M526L	5′-T15-TTGACCCTCAAGCGC-3′	45	60
		RMP-M526P	5′-T15-TTGACCCCCAAGCGC-3′	47	67
		RMP-W531S	5′-T15-CGACTGTCGGCGCTG-3′	50	73
		RMP-M531L	5′-T15-CGACTGTTGGCGCTG-3′	47	67
		RMP-M531W	5′-T15-CCGACTGTGAGCGCT-3′	47	67
		RMP-W533L	5′-T15-GGCGCTGGGGCCCGGC-3′	61	94
		RMP-M533P	5′-T15-GTCGGCGCCGGGGCCCG-3′	64	94
Isoniazid	katG	INH-W315S	5′-T15-ATCACCAGCGGCATC-3′	45	60
		INH-M315T	5′-T15-ATCACCACCGGCATC-3′	45	60
		INH-M315N	5′-T15-ATCACCAACGGCATC-3′	42	53
	inhA	inhA-W	5′-T15-GGCGAGACGATAGGT-3′	57	60
		inhA-M	5′-T15-GGCGAGATGATAGGT-3′	56	52

^aW, wild type; M, mutant. Numbers and letters following W or M refer to amino acid types encoded by the codon at the mutation region.

^bIn each sequence, the underlined letter is the position of the potential mismatch.