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Western blot analysis of Pythium insidiosum P1 CFA probed with S1 to S12 pythiosis sera (lane 1 to 12, respectively) at 1:1,000 dilution. Lanes 13 and 14 represent S12 serum at 1:10,000 and 1:100,000 dilutions, respectively. Predicted sizes are shown in kDa on the left.
