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. 2006 Apr 10;7(6):686–698. doi: 10.1111/j.1600-0854.2006.00420.x

Figure 2. Anisomycin promotes endocytosis of endogenous epidermal growth factor receptor (EGFR).

Figure 2

(A) Control HeLa cells (blue line) or cells treated with EGF (green) or anisomycin (pink) for 15 min were fixed, and the amount of EGFR present at the plasma membrane was measured by flow cytometry. (B) Diagram representing the mean + SD from three independent experiments. Percentage of EGFR at the plasma membrane refers to the fractional reduction of surface receptor in response to 15-min agonist exposure. For all flow cytometry experiments, a minimum of 10 000 cells were counted.