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. 2006 Apr 10;7(6):686–698. doi: 10.1111/j.1600-0854.2006.00420.x

Figure 7. Inactivation of p38 by SB203580 inhibits anisomycin-induced epidermal growth factor receptor (EGFR) internalization.

Figure 7

(A) HeLa cells expressing EGFR-green fluorescent protein (GFP) were left untreated or preincubated with SB203580, dimethyl sulphoxide or AG1748 for 30 min prior to stimulation with anisomycin for 15 min. (B) After 30 min preincubation with SB203580, HeLa cells expressing EGFR-GFP were stimulated with anisomycin for 15 min in the presence of rhodamine transferrin, fixed and analyzed by confocal microscopy. (C) Control cells or cells preincubated with SB203580 for 30 min were treated with anisomycin for 15 min, fixed and the cell surface EGFR measured by flow cytometry. (D) Graphics represents the mean + SD values obtained from two independent experiments. Scale bar represents 10 µm.