TABLE 1.
E. coli strains and plasmids
| Strain or plasmid | Genotype (siderophore[s])a | Source or reference |
|---|---|---|
| E. coli K-12 strains | ||
| DM1187 | lexA51 lexA3 | 50 |
| H5058 | aroB tsx malT cirA fepA fiu (Entb) | 5 |
| MG1655 | F− λ−rph-1 (Ent) | 9 |
| ORN172 | thr-1 leu-6 thi-1 Δ(argF-lac)U169 xyl-7 ara-13 mtl-2 gal-6 rspL tonA2 minA minB Δ(fimEACDFGH)::kan pilG1 | 63 |
| QC2517 | MG1655 recD190::Tn10 Δfur::cat (Ent) | 20 |
| QT1272 | H5058 ΔtonB::kan (Entb) | This study |
| Other E. coli strains | ||
| QT686 | UCB34 Δiha::tetAR(B) (Ent, Aero, Ybtc) | This study |
| QT796 | UCB34 Δfur::cat (Ent, Aero, Ybtc) | This study |
| UCB34 | O17/77 ExPEC CGA isolate (cystitis); possesses F16 papA, papGII, iutA, kpsMTII, fyuA, ybte, iha; lacks sfa/foc, afa/dra, hly, cnf, iroN, iss, malX (Ent, Aero, Ybtc) | Amee Manges, 46 |
| χ7122 | Avian pathogenic E. coli, O78:K80:H9; gyrA Nalr (Ent, Aero, Sal) | 52 |
| Plasmids | ||
| pACYC184 | p15A ori; Tcr Cmr | 14 |
| pAMR18 | Vibrio cholerae CA401 irgA in pACYC184 | 49 |
| pBC SK+ | ColE1 ori, Cmr | Stratagene, La Jolla, CA |
| pBR322 | pMB1 ori; Apr Tcr | 10 |
| pC6 | E. coli rrnB operon in pBR322, Apr | C. Squires |
| pCR2.1-TOPO | pUC ori; Apr Kmr | Invitrogen, Carlsbad, CA |
| pIJ68 | MG1655 fepA in pACYC184, Cmr | This study |
| pIJ82 | UCB34 iha in pCR2.1-TOPO; Apr Kmr | This study |
| pIJ83 | iha::tetAR(B) in pACYC184, Cmr | This study |
| pIJ84 | UCB34 iha in pACYC184, Cmr | This study |
| pIJ111 | UCB34 iha in pBC SK+, Cmr | This study |
| pIJ120 | χ7122 iroN in pBC SK+, Cmr | This study |
| pIJ122 | MG1655 fepA in pBC SK+, Cmr | This study |
| pIJ123 | irgA in pBC SK+, Cmr | This study |
| pIJ159 | H5058 tonB in pBR322; Apr Tcr | This study |
| pKD13 | Rγ ori, FRT-flanked kanamycin resistance; Kmr Apr | 16 |
| pKD46 | pSC101 Ts ori, araBp-gam-bet-exo, Apr | 16 |
| pYA3442 | tetAR(B) in pBSL86, Apr | 19 |
a
Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin; Tc, tetracycline; Ent, enterobactin; Aero, aerobactin; Sal, salmochelin; Ybt, yersiniabactin. The sidephores listed in parentheses are known siderophores produced.
b
Only when supplemented with shikimate or dihydroxybenzoic acid.
c
Possesses yersiniabactin genes, but no product was detected by mass spectrometry.