TABLE 1.
Recombinant detoxified CyaA used in this study
| CyaA toxoid | TB10.4 insert
|
Flanking sequences of TB10.4 inserta | OVA insertb (position) | |
|---|---|---|---|---|
| Fragment | Position | |||
| CyaA-TB10.4:1-96-OVA | 1-96 (entire) | 233 | EATGG232VRVPRRRRKSL-TB10.4-SLRRRRYVH233LDRER | + (108) |
| 336 | KEYIG335VRVPRRRRKSL-TB10.4-SLRRRRYVH336QQRGE | |||
| CyaA-TB10.4:15-33-OVA | 15-33 | 233 | EATGG232VR-AGDMAGYAGTLQSLGAEIA-VH233LDRER | + (108) |
| 336 | KEYIG335VR-AGDMAGYAGTLQSLGAEIA-VH336QQRGE | |||
| CyaA-TB10.4:20-28-OVA | 20-28 | 233 | EATGG232VR-GYAGTLQSL-VH233LDRER | + (108) |
| 336 | KEYIG335VR-GYAGTLQSL-VH336QQRGE | |||
| CyaA-TB10.4:18-30-OVA | 18-30 | 233 | EATGG232VR-MAGYAGTLQSLGA-VH233LDRER | + (108) |
| 336 | KEYIG335VR-MAGYAGTLQSLGA-VH336QQRGE | |||
| CyaA-OVA:257-264 | + (224) | |||
| CyaA | ||||
a
The inserts in CyaA consisted of the entire polypeptide of TB10.4 (residues 1 to 96) or of the indicated TB10.4-derived epitopes, of which the full sequence is underlined. The introduced flanking residues are printed in boldface, and the superscript numbers indicate positions of CyaA residues between which the antigen inserts were introduced.
b
The OVA insert is made up of peptide residues 257 to 264.