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. 2006 Jun;74(6):3347–3354. doi: 10.1128/IAI.01724-05

FIG. 3.

FIG. 3.

Expression of SmLRR in S. mansoni. (A) Reverse transcription-PCR analysis of total RNA extracted from adult worms (lane 1), cercariae (lane 2), and eggs (lane 3) and control RNA obtained from rat spleen cells (lane 4). (B) Western blot analysis of immunoreactivity of rat anti-SmLRR antiserum with S. mansoni antigen. Soluble adult worm (lanes 1 and 4), cercaria (lanes 2 and 5), and egg (lanes 3 and 6) antigens were subjected to SDS-PAGE, transferred to a nitrocellulose membrane, and probed with preimmune serum (1/50) (lanes 1 to 3) or with rat anti-SmLRR antiserum (1/50) (lanes 4 to 6). (C) Coomassie blue-stained gel showing the position of recombinant SmLRR protein.