FIG. 3.

F. tularensis LVS LPS did not antagonize PMN priming by a potent endotoxin when the LBP concentration was reduced to 10 ng/ml, which was limiting. Conditions were otherwise as described for Fig. 2. The results from one experiment are presented in two panels for clarity. A, Penta-acylated LOS from an msbB mutant efficiently antagonized priming by 5 ng/ml of N. meningitidis (Nm) LOS (P < 0.05 for each concentration of msbB LOS in comparison with N. meningitidis LOS at 5 ng/ml). B, F. tularensis (Ft) LVS LPS had no significant effect on priming by 5 ng/ml of N. meningitidis LOS (P > 0.05 for the comparison between each concentration of F. tularensis LVS LPS and N. meningitidis LOS). Results are the averages for sextuplicate samples ± standard errors of the means and are representative of those from three experiments. ALU, arbitrary light units.