FIG. 7.
Comparison of glycosphingolipid recognition of CFA/I and heterologous CF fimbriae of ETEC. Autoradiograms were obtained by binding of 125I-labeled CFA/I fimbriae (A), CS4 fimbriae (B), and CS7 fimbriae (C) to serial dilutions (0.4 to 2.0 μg) of glycosphingolipids in a chromatogram binding assay. The binding assay was done as described in Materials and Methods. The solvent system used was chloroform-methanol-water (60:35:8, by volume). Autoradiography was performed for 12 h. Lanes: 1 to 4, glucosylceramide (Glcβ1Cer), isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer), and gangliotetraosylceramide (Galβ3GalNAcβ4Galβ4Glcβ1Cer) (0.4 to 2.0 μg of each compound); 5 to 8, lactosylceramide (Galβ4Glcβ1Cer) with t18:0-h16:0-h24:0, neolactotetraosylceramide (Galβ4GlcNAcβ3Galβ4Glcβ1Cer), and Lea pentaglycosylceramide [Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer] (0.4 to 2.0 μg of each compound).