FIG. 8.

Binding of CFA/I fimbriae of ETEC to nonacid glycosphingolipids of human small intestine. The glycosphingolipids were separated on aluminum-backed silica gel plates and visualized with anisaldehyde (A). Duplicate chromatograms were incubated with ¹²⁵I-labeled CFA/I fimbriae (B) and monoclonal antibodies directed against the Le^a determinant (C), followed by autoradiography for 12 h, as described in Materials and Methods. The solvent system used was chloroform-methanol-water (60:35:8, by volume). Lanes: 1 to 3, nonacid glycosphingolipids of human small intestine of three different individuals (40 μg/lane); 4, reference neolactotetraosylceramide (Galβ4GlcNAcβ3Galβ4Glcβ1Cer) (4 μg); 5, reference galactosylceramide (Galβ1Cer) (4 μg); 6, reference Le^a pentaglycosylceramide [Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer] (4 μg).