TABLE 1.
Binding of 125I-labeled native CFA/I fimbriae, 35S-labeled recombinant CFA/I-expressing E. coli Top10-CFA/I, and 125I-labeled CFA/I fimbriae with deletion of the CfaE subunit (CFA/I/E−) to pure glycosphingolipids on thin-layer chromatograms
| Compound no., trivial name | Structure | CFA/I fimbriae | E. coli Top10-CFA/I | CFA/I/E− fimbriae |
|---|---|---|---|---|
| Simple compounds | ||||
| 1, Galactosylceramide | Galβ1Cer | −a | − | − |
| 2, Glucosylceramide | Glcβ1Cer | + | + | + |
| 3, Sulfatide (d18:1-16:0 and 24:0)b | SO3-Galβ1Cer | − | − | NDc |
| 4, LacCer (d18:1-16:0-24:0) | Galβ4Glcβ1Cer | − | − | − |
| 5, LacCer (t18:0-h16:0-h24:0) | Galβ4Glcβ1Cer | + | + | + |
| Ganglioseries | ||||
| 6, GgO3 (d18:1-16:0 and 24:0) | GalNAcβ4Galβ4Glcβ1Cer | + | + | + |
| 7, GgO4 (t18:0-h16:0 and h24:0) | Galβ3GalNAcβ4Galβ4Glcβ1Cer | + | + | + |
| Neolactoseries | ||||
| 8, Neolactotetra (d18:1-16:0 and 24:1) | Galβ4GlcNAcβ3Galβ4Glcβ1Cer | + | + | + |
| 9, H5-2 (d18:1-16:0/24:0) | Fucα2Galβ4GlcNAcβ3Galβ4Glcβ1Cer | + | + | + |
| 10, Lex-5 (t18:0-h16:0-h24:0) | Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer | + | + | + |
| 11, B5 (d18:1-16:0/24:0) | Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer | + | + | + |
| 12, Ley-6 (t18:0-h16:0-h24:0) | Fucα2Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer | + | + | + |
| 13, B6-2 (d18:1-16:0/24:0) | Galα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | − | − |
| 14, A6-2 (d18:1-16:0/24:0) | GalNAcα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | − | ND |
| 15, A7-2 | GalNAcα3(Fucα2)Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer | − | − | − |
| 16, Neolactohexa (d18:1-16:0-24:0) | Galβ4GlcNAcβ6(Galβ4GlcNAcβ3)Galβ4Glcβ1Cer | − | − | ND |
| Lactoseries | ||||
| 17, Lea-5 (t18:0-h16:0-h24:0) | Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer | + | + | + |
| 18, Leb-6 (t18:0-h16:0-h24:0) | Fucα2Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer | − | − | − |
| 19, A7-1 (t18:0-h16:0-h24:0) | GalNAcα3(Fucα2)Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer | − | − | − |
| 20, B7-1 | Galα3(Fucα2)Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer | − | − | ND |
| Globoseries | ||||
| 21, Globotri (d18:1-16:0 and 24:0) | Galα4Galβ4Glcβ1Cer | − | − | − |
| 22, Isoglobotri (t18:0-h22:0 and h24:0) | Galα3Galβ4Glcβ1Cer | + | + | + |
| 23, Globotetra (d18:1-16:0 and 24:0) | GalNAcβ3Galα4Galβ4Glcβ1Cer | − | − | − |
| 24, Isoglobotetra (d18:1-16:0 and 24:0) | GalNAcβ3Galα3Galβ4Glcβ1Cer | − | − | − |
| 25, Forssman (d18:1-16:0 and 24:0) | GalNAcα3GalNAcβ3Galα4Galβ4Glcβ1Cer | − | − | |
| 26, (t18:0-h22:0 and h24:0) | Galα3Galα3Galβ4Glcβ1Cer | + | + | + |
| Gangliosides | ||||
| 27, GM3 (d18:1-18:0/d20:1-18:0) | NeuAcα3Galβ4Glcβ1Cer | − | − | ND |
| 28, GM2 | GalNAcβ4(NeuAcα3)Galβ4Glcβ1Cer | − | − | ND |
| 29, GD2 | GalNAcβ4(NeuGcα8NeuGcα3)Galβ4Glcβ1Cer | − | − | ND |
| 30, GD3 | NeuAcα8NeuAcα3Galβ4Glcβ1Cer | − | ND | ND |
| 31, GM1 (d18:1-18:0 and d18:1-20:0) | Galβ3GalNAcβ4(NeuAcα3)Galβ4Glcβ1Cer | − | − | ND |
| 32, GD1b (d18:1-18:0/d20:1-18:0) | Galβ3GalNAcβ4(NeuAcα8NeuAcα3)Galβ4Glcβ1Cer | − | − | ND |
| 33, NeuAcα3SPG (d18:1-16:0/24:0) | NeuAcα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | − | ND |
| 34, NeuGcα3SPG | NeuGcα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | ND | ND |
| 35, NeuAcα6SPG | NeuAcα6Galβ4GlcNAcβ3Galβ4Glcβ1Cer | − | − | ND |
| 36, NeuAcα-Lea | NeuAcα3Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer | − | − | ND |
| 37, NeuAcα-Lex | NeuAcα3Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer | − | − | ND |
| 38 | Galβ4GlcNAcβ6(NeuAcα6Galβ4GlcNAcβ3)Galβ4Glcβ1Cer | − | − | ND |
Binding is defined as follows: +, binding when 4 μg of the glycosphingolipid was applied to the thin-layer chromatogram; −, no binding even at 4 μg.
In the shorthand nomenclature for fatty acids and bases, the number before the colon refers to the carbon chain length and the number after the colon gives the total number of double bonds in the molecule. Fatty acids with a 2-hydroxy group are denoted by the prefix h before the abbreviation, e.g., h16:0. For long-chain bases, d denotes dihydroxy and t denotes trihydroxy. Thus, d18:1 is sphingosine (1,3-dihydroxy-2-aminooctadecene) and t18:0 is phytosphingosine (1,3,4-trihydroxy-2-aminooctadecene).
ND, not determined.