FIG. 2.

Time course of induction of ICAM-1 expression on HBMEC. (A) Surface ICAM-1 expression on confluent HBMEC monolayers after incubation with Pf-IRBC (P. falciparum 3D7; 0.6 × 10⁸ cells/well) for different times. The P value was <0.05 for all times compared to the medium control (n = 4). (B) Time course of binding of Pf-IRBC (P. falciparum 3D7; 0.6 × 10⁸ cells/well) to confluent HBMEC monolayers. The P value was <0.05 for comparisons of different times (n = 4). No binding was observed with control RBC. (C) Reversibility of ICAM-1 expression after incubation of confluent HBMEC monolayers with Pf-IRBC (0.6 × 10⁸ cells/well) for 6 h compared with the results for TNF-α. Subsequently, Pf-IRBC and TNF-α were removed, and regression of the induced surface ICAM-1 expression was assessed for up to 72 h. The P value was <0.05 for all times compared to the medium control (n = 4).