FIG. 1.
Regulated expression of CCL20 by Campylobacter jejuni-infected T84 cell monolayers. A. RT-PCR analysis of CCL20 from total RNA isolated from T84 epithelial cells inoculated with C. jejuni strain 81-176 at multiplicities of infection of 25:1, 50:1, and 100:1. RNA from uninfected cells (Ctrl) or cells treated with 20 ng/ml TNF-α were used as negative and positive controls, respectively. Reactions performed in the absence of RNA (no RNA) or with RNase-free water (H2O) served as negative controls. GAPDH verified equal loading between samples. Results are representative of three independent experiments. B. Kinetics of chemokine mRNA expression in T84 cells in response to C. jejuni infection. Epithelial cells were infected with C. jejuni strain 81-176 at an MOI of 25:1 (solid circle), 50:1 (solid triangle), or 100:1 (solid square). Total RNA was isolated 6, 12, and 24 h after C. jejuni inoculation of T84 epithelial cells and CCL20 mRNA expression assessed using real-time PCR. Samples were compared to uninfected control (empty circle), which was designated baseline. The 
comparative CT method was used to define relative gene expression. Values are the means for triplicate samples and are representative of two separate experiments.